Effect of Solanum Melongena peel anthocyanin extracts in Lipase Activity which purified from TI-diabetes patients
Abstract
Lipase is a crucial enzyme regulating triglyceride metabolism in the blood muscle and adipose tissue. lipase was purified from the blood of the patients with type 1 diabetes using a range of techniques including precipitation with ammonium sulfate, dialysis, and ion exchange chromatography by CM-cellulose. The specific activity was found to be 0.0117, 0.0252, and 0.1035 U/mg protein, respectively, purification fold was found to be 1.88, 4.06, and 16.96, respectively. Molecular weight was 76.5 KD. The research also examined the optimal conditions for the enzyme, including the effect of enzyme concentration, temperature, pH, and substrate. The optimal conditions were found to be 100 μl of enzyme at 37℃, 7.2, and 1.2, respectively. Anthocyanin extracts were isolated from eggplant peel by a mixture of solvents (HCL, Methanol, chloroform) identification and estimation of these compounds by HPLC, total and anthocyanin compounds Cyanidin Delphinidin Malvidin Peonidin 439.25 64.35, 215.15, 79.9, 79.85 μg/kg of eggplant peer respectively. The extracts exhibited competitive inhibition of LPL activity at concentrations of 20 and 40 ppm.
Keywords:
Anthocyanin, fatty acids lipid profile, atherogenic index, diabetes mellitus.
How to Cite
Zena Ismail Abdullah, & Nashwan Ibrahem Al-Lehebe. (2024). Effect of Solanum Melongena peel anthocyanin extracts in Lipase Activity which purified from TI-diabetes patients. Bani Waleed University Journal of Humanities and Applied Sciences, 9(3), 1-11. https://doi.org/10.58916/jhas.vi.293
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References
1.
Abbas, Z. A.-E., & El-Yassin, H. D. (2022). The impact of glycemic control on procalcitonin level in patients with type ii diabetes. Medical Journal of Babylon, 19(3), 391-395.
2.
Aronson, R., Goldenberg, R., Boras, D., Skovgaard, R., & Bajaj, H. (2018). The Canadian hypoglycemia assessment tool program: insights into rates and implications of hypoglycemia from an observational study. Canadian journal of diabetes, 42(1), 11-17.
3.
Baynes, H. W. (2015). Classification, pathophysiology, diagnosis and management of diabetes mellitus. J diabetes metab, 6(5), 1-9.
4.
Befani, O., Grippa, E., Saso, L., Turini, P., & Mondovi, B. (2001). Inhibition of monoamine oxidase by metronidazole. Inflammation Research, 50(2), 136-137.
5.
Cao, G., Sofic, E., & Prior, R. L. (1996). Antioxidant capacity of tea and common vegetables. Journal of Agricultural and Food Chemistry, 44(11), 3426-3431.
6.
Chen, T.-Z., Xie, S.-L., Jin, R., & Huang, Z.-M. (2014). A novel lipoprotein lipase gene missense mutation in Chinese patients with severe hypertriglyceridemia and pancreatitis. Lipids in Health and Disease, 13, 1-5.
7.
Dhalaria, R., Verma, R., Kumar, D., Puri, S., Tapwal, A., Kumar, V., . . . Kuca, K. (2020). Bioactive compounds of edible fruits with their anti-aging properties: A comprehensive review to prolong human life. Antioxidants, 9(11), 1123.
8.
do Nascimento, R. d. P., & da Fonseca Machado, A. P. (2023). The preventive and therapeutic effects of anthocyanins on colorectal cancer: a comprehensive review based on up-to-date experimental studies. Food Research International, 170, 113028.
9.
Fonseca, V., Berger, L., Beckett, A., & Dandona, P. (1985). Size of pancreas in diabetes mellitus: a study based on ultrasound. British medical journal (Clinical research ed.), 291(6504), 1240.
10.
HAYASHI, R., TAJIMA, S., & YAMAMOTO, A. (1986). Purification and characterization of lipoprotein lipase from human postheparin plasma and its comparison with purified bovine milk lipoprotein lipase. The Journal of Biochemistry, 100(2), 319-331.
11.
Junglee, D., De Albarran, R., Katrak, A., Freedman, D., Barradas, M., Beckett, A., & Dandona, P. (1982). Low Pancreatic Lipase in Insulin Dependent Diabetics. Clinical Science, 63(3), 67P-67P.
12.
Kavitha, V., Revathi, N., & Kingsley, S. (2008). Biology of brinjal pest, Leucinodes orbonalis Guen. of Erode region in Tamil Nadu. Journal of Entomological Research, 32(3), 255-257.
13.
Meana, C., Rubin, J. M., Bordallo, C., Suarez, L., Bordallo, J., & Sanchez, M. (2016). Correlation between endogenous polyamines in human cardiac tissues and clinical parameters in patients with heart failure. Journal of cellular and molecular medicine, 20(2), 302-312.
14.
Merecz-Sadowska, A., Sitarek, P., Kowalczyk, T., Zajdel, K., Jęcek, M., Nowak, P., & Zajdel, R. (2023). Food anthocyanins: Malvidin and its glycosides as promising antioxidant and anti-inflammatory agents with potential health benefits. Nutrients, 15(13), 3016.
15.
Mohammadi, N., Farrell, M., O’Sullivan, L., Langan, A., Franchin, M., Azevedo, L., & Granato, D. (2024). Effectiveness of anthocyanin-containing foods and nutraceuticals in mitigating oxidative stress, inflammation, and cardiovascular health-related biomarkers: a systematic review of animal and human interventions. Food & Function.
16.
Nair, M. (2007). Diabetes mellitus, part 1: physiology and complications. British journal of nursing, 16(3), 184-188.
17.
Neff, M. M., & Chory, J. (1998). Genetic interactions between phytochrome A, phytochrome B, and cryptochrome 1 during Arabidopsis development. Plant physiology, 118(1), 27-35.
18.
Newman, C., Egan, A. M., Ahern, T., Al-Kiyumi, M., Bacon, S., Bahaeldein, E., . . . Brosnan, E. (2022). Retrospective national cohort study of pregnancy outcomes for women with type 1 and type 2 diabetes mellitus in Republic of Ireland. Diabetes research and clinical practice, 189, 109947.
19.
Noda, Y., Kneyuki, T., Igarashi, K., Mori, A., & Packer, L. (2000). Antioxidant activity of nasunin, an anthocyanin in eggplant peels. Toxicology, 148(2-3), 119-123.
20.
Okunwaye, T., Obibuzor, J., & Okogbenin, E. (2015). Purification and biochemical properties of lipase from raphia palm fruit mesocarp. African Journal of Biochemistry Research, 9(5), 73-80.
21.
Pirahanchi, Y., Anoruo, M., & Sharma, S. (2023). Biochemistry, lipoprotein lipase. In StatPearls [Internet]: StatPearls Publishing.
22.
Różańska, D., & Regulska-Ilow, B. (2018). The significance of anthocyanins in the prevention and treatment of type 2 diabetes. Adv Clin Exp Med, 27(1), 135-142.
23.
Savych, A., & Marchyshyn, S. (2021). Inhibition of pancreatic lipase by water extracts of some herbal mixtures. Methods, 20, 21.
24.
Sharma, N., Sharma, R., Rajput, Y. S., Mann, B., Singh, R., & Gandhi, K. (2021). Separation methods for milk proteins on polyacrylamide gel electrophoresis: Critical analysis and options for better resolution. International Dairy Journal, 114, 104920.
25.
Shim, Y.-S., Kim, S., Seo, D., Park, H.-J., & Ha, J. (2014). Rapid method for determination of anthocyanin glucosides and free delphinidin in grapes using u-HPLC. Journal of Chromatographic Science, 52(7), 629-635.
26.
Waterborg, J. H. (2009). The Lowry method for protein quantitation. The protein protocols handbook, 7-10.
27.
Wei, X., Wang, D., Yang, Y., Xia, M., Li, D., Li, G., . . . Ling, W. (2011). Cyanidin‐3‐O‐β‐glucoside improves obesity and triglyceride metabolism in KK‐Ay mice by regulating lipoprotein lipase activity. Journal of the Science of Food and Agriculture, 91(6), 1006-1013.
28.
Zhao, Z., Yan, H., Zheng, R., Khan, M. S., Fu, X., Tao, Z., & Zhang, Z. (2018). Anthocyanins characterization and antioxidant activities of sugarcane (Saccharum officinarum L.) rind extracts. Industrial Crops and Products, 113, 38-45.
29.
Zimmet, P. (2003). The burden of type 2 diabetes: are we doing enough? Diabetes & metabolism, 29(4), 6S9-6S18.
