Isolation, Identification of Candida Species in Diarrhea Patients: From Zintan City, Libya

  • Adel,M. Jama Departmen of Zoology, Faculty of Science, University of zintan, zintan, Libya
  • Farhat Abouzkhar Department of Microbiology,, Faculty of Science, University of zintan, zintan, Libya
  • Haneen Madi Department of Microbiology,, Faculty of Science, University of zintan, zintan, Libya
  • Nasrin Salem Department of Microbiology,, Faculty of Science, University of zintan, zintan, Libya
DOI https://doi.org/10.58916/jhas.v10i2.727
منشور: 2025-04-15
305
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الملخص

 Candida species are significant fungal pathogens responsible for various infections, including gastrointestinal Candidiasis. This study aimed to investigate the prevalence of Candida species in diarrhea patients and explore their association with age and gender in Zintan City, Libya. Materials and Methods: A total of 102 stool samples were collected from patients with gastrointestinal symptoms. Samples were cultured on Sabouraud’s dextrose agar (SDA), potato dextrose agar (PDA), and Chrom Agar for species identification. Direct microscopic examination and germ tube tests were performed to confirm Candida species. Statistical analysis was conducted to assess the distribution of infections across age and gender groups. Results: The study revealed a Candida infection rate of 44.12%, with C. albicans being the most prevalent species (44.44%), followed by  C. glabrata (31.11%). The highest infection rate was observed in the 1–10 years age group (53.33%), while no infections were reported in the 61–70 and 81–90 years age groups. Regarding gender distribution, 58% of infections were found in women, and 42% in men, with no significant gender-based differences (p > 0.05). Conclusion: This study highlights the significant role of Candida, particularly C. albicans, in gastrointestinal infections among diarrhea patients. The high prevalence in children underscores their vulnerability, while the absence of gender-based differences emphasizes the importance of non-gender-related risk factors. The findings advocate for increased clinical awareness and further research to improve diagnostic and treatment strategies for Candida-associated diarrhea.

الكلمات المفتاحية: Candidiasis; C. albicans; DIARRHEA; GASTROINTESTINAL CANDIDIASIS; LIBYA

كيفية الاقتباس

Adel,M. Jama, Farhat Abouzkhar, Haneen Madi, & Nasrin Salem. (2025). Isolation, Identification of Candida Species in Diarrhea Patients: From Zintan City, Libya. مجلة جامعة بني وليد للعلوم الإنسانية والتطبيقية, 10(2), 138-146. https://doi.org/10.58916/jhas.v10i2.727
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الرخصة

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السيرة الشخصية للمؤلف

  • Adel,M. Jama، Departmen of Zoology, Faculty of Science, University of zintan, zintan, Libya

    Name: Adel Jama
    Date of Birth: April, 1973.
    Nationality: Libyan.
    Sex: Male.
    Education from high: High school (from 1988-1991)
    School Higher education (Bachelor of Veterinary Medicine), (1991-1996).
    Work Address: Department of Zoology, Faculty of science, AL- Zintan University, LIBYA.
    E-mail address: adel.jama@uoz.edu.ly /// adelj73828@yahoo.com
    Employment History:
    1. Vet, hospital of Veterinary Medicine, Om-Algersan, Libya. (1998-2005)
    2. Assistant Lecturer of Histology and Physiology, Faculty of Science, AL-Jabal-Algharbi University, Libya. (2006-2007).
    3. Lecturer of Biology. Faculty of Science, Zoology Department, University of Al Jabal Al Gharb. Zintan-Libya. (2014-2017).
    4. Assistant Professor, Faculty of Science, Zoology Department, University of Al Jabal Al Gharb. Zintan-Libya. (2017-2021).
    5. Head of Department of Zoology, Faculty of Science. , University of Zintan.(2016-2022)
    6. Head of Department of Microbiology, Faculty of Science. , University of Zintan.(2023)


    Academic Qualifications:
    1.B.V.Sc. in Veterinary Medical Sciences, AL-Jabal-Algharbi University, 1996.
    2.M.V.Sc. in Biology, 7th April University, 2006.
    3. Course in PCR, at institute for plant protection and environment. Belgrade, Serbia 2013.
    4.Ph.D. in Biology, Faculty of Biology, University of Belgrade, Belgrade, Serbia. (2013).
    5. Assistant Professor, Faculty of Science. , University of Zintan.2017
    6. Associate Professor, Faculty of Science. , University of Zintan. 2021-2025
    Conference contributions:
    1- Veterinary Medical Conference Maghreb held on 3-5 April 1998, at Tripoli, Libya
    2- Arab environmental conference held on 7-10 December 2007 at Cairo, Egypt. Jama ; F. Lashein; M. Almalah: (studying a vital role of olive oil on cadmium induced toxicity in rabbits)
    3- Olive trees, healthy and medical conference held on March 2007 at AL-Jabal-Algharbi University, Garian, Libya.
    A. Jama ; F. Lashein; M. Almalah: (proactive effect of olive oil in some physiological parameters agonist cadmium toxicity in rabbits).
    4- International symposium one hundred years of Ivan Djajas (Jean Giaja) Belgrade school of physiology held on 10-14 September 2010 at University of Belgrade, Belgrade, Serbia.
    5- EEMS, Europen environmental – mutagen society- held on 4-7 July 2011, at Fira palace hotel, Barcelona, ​​Spain. Evaluation of the effects of probiotics on cadmium induced genotoxicity. P. 024
    6- The seventh Libyan conference for medical sciences. held on 11-13 /10/2022 at Zawia. Isolation of Klebisella bacteria from patients with urinary tract infection and determination of its sensitivity to antibiotic in the Zintan region of the Western Mountains- Libya
    Publications:
    1- ADEL M. JAMA, DRAGANA MITIĆ-ĆULAFIĆ, S. KOLAREVIĆ, SINIŠA F. ĐURAŠEVIĆ and JELENA KNEŽEVIĆ-VUKČEVIĆ: Protective effect of probiotic bacteria against cadmium induced genotoxicity in rat hepatocytes in vivo and in vitro. Arch. Biol. Sci., Belgrade, 64 (3), 1197-1206, 2012.
    2- Jama, A.M., 2013. Useful information on the model used today (Doctoral dissertation, University of Belgrade (Serbia).
    3- Jama, A.M., Shukshuk, A.H. and Erhouma, E.A., 2015. ENTAMAEBA HISTOLYTICA INFECTION IN RELATION TO SOME BIOFACTORS. Journal of Basic Sciences, 27, pp.41-52.

    4- Sinisa Djurasevic, Adel Jama, Nebojsa Jasnic, Predrag Vujovic, Milos Jovanovic. Dragana Mitic-Culafic, Jelena Knezevic-Vukcevic, Maja Cakic-Milosevic, Konstantin Ilijevic, and Jelena Djordjevic. The Protective Effects of Probiotic Bacteria on Cadmium Toxicity in Rats. J. Med Food 20 (2) 2017, 189–196
    5- Esadek Ali Erhouma, Adel Jama. Study of the genetic variability in the gag gene of the lentivirus. Al-Jabal Scientific Journal. (1) 1-19, 2016.
    6- Adel Masoud Jama, Maren Haqiq, Aya Zriba, Ibtihal Al-Jaafari. A Study of the Prevalence of Amoebic Dysentery and its Relationship to Some Environmental Factors in the Yafran Region. Sada Al-Ma'rifa Journal, Issues (3-4), 251–266, 2015.
    7- Zahra Saleh, Adel Jama, Al-Sadiq Rahoma. A statistical study of cutaneous leishmaniasis cases reported to the National Center for Disease Control, Yafran Branch, during the years 2013-2016. The First Conference on Applied Sciences. Faculty of Science, Zintan. 31-38, 2017.
    8- Adel Jama, Riad Alhodiry. Prevalence of Trichomoniasis in Domestic Pigeons. Aljabal Scientific Journal. (2) 221-227, 2017.
    9- Ali Amer Ali Al-Arabi, Adel Masoud Jami. A study of the prevalence of some intestinal parasites among patients attending hospitals in Zintan, Umm Al-Jarsan, and Yafran in the Western Mountains region. Journal of Faculties of Education, University of Zawiya. Issue (13), pp. 276-289, 2019.
    10- Adel Jama, Riad Alhodiry, Ali Al-Arabi, Alkasm Shukshuk. Prevalence of ectoparasite in indigenous chicken farm at Al-Jabal Al-Gharby, Libya. Journal of Humanities and Applied Sciences (JHAS). (32) 88-98 (2019).
    11- Widad Al-Malal, Fidaa Amoush, Aisha Omar, Hoda Al-Sharif, Fatima Abu Zalma, Adel Jamea. A preliminary study on the inhibitory effect of rosemary leaf extracts.

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