BIOFORTIFICATION OF WHEAT PRODUCTIVITY USING HUMIC ACID, ZINC AND AMINO ACIDS APPLICATION
Abstract
Soil application of humic acids (HA) and foliar fertilization with bio-stimulants as Zn and amino acids, and others can affect plant growth by enhancing resistance to abiotic stress and raising and sustainably increasing grain yield. Consequently, this investigation was carried out to test the response of Bohoth-210 wheat variety to soil-application of HA and foliar application of Zn and amino acids (AA) and their interaction on yield and yield components during the cropping season of 2023/2024. The factorial experiment was designed according to a complete randomized block design (CRBD) in two factors. The first factor was the application of HA as a soil amendment, with variable levels of application set at 0, 7, and 14 kg/ha. The second factor was the foliar spraying levels of (water as a control, Zn at a rate of 1.5 kg/ha, amino acids at a concentration of 1 cm/liter of water), and a combination treatment of Zn and amino acids. These treatments were randomly distributed in three replicates. The findings observed that the application of HA, Zn, and amino acids, along with their interactions, particularly at higher levels has a significant influence on yield characteristics as grain yield, yield components, and the overall protein content of the wheat produced. Such results underscore the significance of utilizing these agricultural inputs effectively to enhance wheat production in Libya, thereby contributing to food security.
Keywords:
wheat; yield; yield components; zinc
amino acids; humic acid applications
How to Cite
Seham Mohamed Alzweek, & Radia Omar Salem. (2025). BIOFORTIFICATION OF WHEAT PRODUCTIVITY USING HUMIC ACID, ZINC AND AMINO ACIDS APPLICATION. Bani Waleed University Journal of Humanities and Applied Sciences, 10(1), 289-301. https://doi.org/10.58916/jhas.v10i1.677
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References
1.
Abd El-Aal, F. S., Shaheen, A. M., Ahmed, A. A., and Mahmoud, A. R. (2010). Effect of foliar application of urea and amino acids mixtures as antioxidants on growth, yield and characteristics of squash. Res. J. Agric. Biol. Sci, 6(5), 583-588.
2.
Abd El-Aziz, A., Mahmoud, A., Salem, A. H., Aly, R. M., El-Maaboud, A., and Sh, M. (2018). Grain Quality and Protein Yield of Three Bread Wheat Cultivars as Affected by Some Humic Acid and Compost Fertilizer Treatments under Newly Sandy Soil Conditions. Zagazig Journal of Agricultural Research, 45(3), 809-819.
3.
Adiloglu, A., and Adiloglu, S. (2006). The effect of boron (B) application on the growth and nutrient contents of maize in zinc (Zn) deficient soils. Research Journal of Agriculture and Biological Sciences, 2(1), 1-4.
4.
Akinremi, O. O., Janzen, H. H., Lemke, R. L., and Larney, F. J. (2000). Response of canola, wheat and green beans to leonardite additions. Canadian Journal of Soil Science, 80(3), 437-443.
5.
Al-Juthery, H. W., Lahmoud, N. R., Alhasa, A. S., N. A.-J., & Houria, A. (2022). Nano-Fertilizers as a Novel Technique for Maximum Yield in Wheat Biofortification (Article Review). IOP Conference Series: Earth and Environmental Science, 1060, 1-26.
6.
AOAC, A. (1995). Official methods of analysis 16th Ed. Association of official analytical chemists. Washington DC, USA. Sci. Educ.
7.
Arif, M., Tasneem, M., Bashir, F., Yaseen, G. and Anwar, A. (2017). Evaluation of different levels of potassium and zinc fertilizer on the growth and yield of wheat. Int. J. of Biosensors and Bioelectronics, 3(2):242‒246.
8.
Ashmead, H.D. (1986). The absorption mechanism of amino acid chelates by plant cells. In: Foliar feeding of plants with amino acid chelates. Noyes Publications, Park Ridge, New Jersey, USA), pp 219-235
9.
Bakry, B. A., Elewa, T. A., El-Kramany, M. F., and Wali, A. M. (2013). Effect of humic and ascorbic acids foliar application on yield and yield components of two wheat cultivars grown under newly reclaimed sandy soil. International Journal of Agronomy and Plant Production, 4(6), 1125-1133.
10.
Cakmak, I. (2008). Enrichment of cereal grains with zinc: agronomic or genetic biofortification?. Plant and soil, 302(1), 1-17.
11.
Celik, H., Katkat, A. V., Aşık, B. B., and Turan, M. A. (2010). Effects of humus on growth and nutrient uptake of maize under saline and calcareous soil conditions.
12.
CoStat-Cohort Software (2005). CoStat User Manual, version 3 Cohort Tucson, Arizona, USA.
13.
De Melo, B. A. G., Motta, F. L., and Santana, M. H. A. (2016). Humic acids: Structural properties and multiple functionalities for novel technological developments. Mater. Sci. Eng. C 62, 967–974. doi: 10.1016/j.msec.2015.12.001
14.
Ebrahimi, M., Roozbahani, A., and Baghi, M. (2014). Effect of potash fertilizer and amino acids on yield components and yield of maize (Zea mays L.). Crop Research (0970-4884), 48.
15.
El-Dahshouri, M. F. (2017). Effect of zinc foliar application at different physiological growth stages on yield and quality of wheat under sandy soil conditions. Agricultural Engineering International: CIGR Journal, 193-200.
16.
El-Habbasha, E. S., Badr, E. A., and Latef, E. A. (2015). Effect of zinc foliar application on growth characteristics and grain yield of some wheat varieties under Zn deficient sandy soil condition. International Journal Chemtech Research, 8(6), 452-458.
17.
El-Metwally, A., Khalil, N. A., El-Fouly, M. M., and El-Dahshouri, M. F. (2012). Growth, nutrients uptake and grain yield of some wheat cultivars as affected by zinc application under sandy soil conditions. Journal of Plant Production, 3(5), 773-783.
18.
El-Naggar, E.M. and A.M. El-Ghamry (2007). Effect of bio and chemical nitrogen fertilizers with foliar of humic and amino acids on wheat. J. Agric. Sci. Mansoura Univ., 32 (5): 4029-4043.
19.
Esfandiari, E., M. Abdoli, S. B. Mousavi, and B.Sadeghzadeh, 2016. Impact of foliar zinc application onagronomic traits and grain quality parameters of wheat grown in zinc deficient soil. Indian J. of Plant Physiology, 21(3): 263–270.
20.
Faraj, F. S., Ismail, F., & Ab-Rahim, R. (2022). Determinants of Wheat Production in Libya. International Journal of Academic Research in Business and Social Sciences, 10(12), 178- 191.
21.
FAOSTAT (2021). URL: http://www.fao.Org/faostat/en/-data/QC. Food and agriculture organization of the United Nations (FAO). Accessed on 25.
22.
Firdous, S., Agarwal, B. K., and Chhabra, V. (2018). Zinc-fertilization effects on wheat yield and yield components. Journal of Pharmacognosy and Phytochemistry, 7(2), 3497-3499.
23.
Gollenbeek, L., and Van Der Weide, R. (2020). Prospects for Humic Acid Products From Digestate in the Netherlands. Report WPR-867, 5–40. doi: 10.18174/541280.
24.
Gomez, A.K. and Gomez, A.A. 1984. Statistical procedure for agricultural research.2nd edition John Wiley and Sons, Inc., New York
25.
Habib, M. (2009). Effect of foliar application of Zn and Fe on wheat yield and quality. African Journal of Biotechnology, 8(24).
26.
Hassanein, M. S., Zaki, N. M., and Ahmed, A. G. (2019). Effect of Zn foliar application on growth and yield characteristics of two wheat cultivars. Current Science International, 8(3), 491-498.
27.
Hotz, C., and Brown, K. H. (2004). Assessment of the risk of zinc deficiency in populations and options for its control.Food and Nutrition Bulletin, 2: 194–204.
28.
Jackson, M. L. (1973). Soil chemical analysis prentice Hall. Inc., Englewood Cliffs, NJ 498, 183–204.
29.
Kandil, A. A., Sharief, A. E. M., Seadh, S. E., and Altai, D. S. K. (2016). Role of humic acid and amino acids in limiting loss of nitrogen fertilizer and increasing productivity of some wheat cultivars grown under newly reclaimed sandy soil. Int. J. Adv. Res. Biol. Sci, 3(4), 123-136.
30.
Kandil, E. E., and Marie, Eman, A. (2017). Response of some wheat cultivars to nano-, mineral fertilizers and amino acids foliar application. Alex. Science Exchange Journal, 38(January-March), 53-68.
31.
Khalid, A., Hameed, A., & Tahir, M. F. (2023). Wheat quality: A review on chemical composition, nutritional attributes, grain anatomy, types, classification, and function of seed storage proteins in bread making quality. Frontiers in Nutrition, 10, 1-14.
32.
Khan, R. U., Rashid, A., Khan, M. S., and Ozturk, E. (2010). Impact of humic acid and chemical fertilizer application on growth and grain yield of rainfed wheat (Triticum aestivum L.). Pakistan Journal of Agricultural Research, 23(3-4).
33.
Laware, D. R. D. S., and Apparao, B. J. (2010). Effect of organically chelated micronutrients on growth and productivity in okra.Asian J.Exp.Biol.Sci.Spl. 115-117.
34.
Malik, K. A., and Azam, F. (1985). Effect of humic acid on wheat (Triticum aestivum L.) seedling growth. Environmental and Experimental Botany, 25(3), 245-252.
35.
Nardi, S., Schiavon, M., and Francioso, O. (2021). Chemical structure and biological activity of humic substances define their role as plant growth promoters. Molecules 26, 2256. doi: 10.3390/molecules26082256
36.
Olsen, S. (1982). Phosphorus. Methods soil Anal. 2, 403–430.
37.
Pandey, N., G. C. Pathak, and C. P. Sharma, 2006. Zinc is critically required for pollen function and fertilization in lentil. J. of Trace Elements in Medicine and Biology, 20: 89–96.
38.
Sánchez-Palacios, J. T., David Henry, B. P., & Bell, R. (2023). Formulation of zinc foliar sprays for wheat grain biofortification: a review of current applications and future perspectives. Frontiers in Plant Science, 14, 1-14.
39.
Sandhu, R., Chaudhary, N., Bindia, R. S., Singh, K., & Pandey, V. K. (2023). A critical review on integrating bio fortification in crops for sustainable agricultural development and nutritional security. Journal of Agriculture and Food Research, 14, 1-10.
40.
Shetta, D.N., and Zayed, Z. M. (2016). Responses of Acacia gerrardii and Vachelliaorigena seedlings to mineral fertilization and salinity stress in Saudi Arabia. Alexandria Science Exchange Journal, 37(July-September), 430-439.
41.
Sible, C. N., Seebauer, J. R., and Below, F. E. (2021). Plant biostimulants: a categorical review, their implications for row crop production, and relation to soil health indicators. Agronomy 11, 1297. doi: 10.3390/agronomy110 71297
42.
Tahir, M. M., Khurshid, M., Khan, M. Z., Abbasi, M. K., and Kazmi, M. H. (2011). Lignite-derived humic acid effect on growth of wheat plants in different soils. Pedosphere, 21(1), 124-131.
43.
Topp, G. C., Galganov, Y. T., Ball, B. C., and Carter, M. R. (1993). Soil water desorption curves. Soil Sampl. Methods Anal., 569–579.
44.
Van Tol de Castro, T. A., García, A. C., Tavares, O. C. H., Pereira, E. G., de Souza, C. D. C. B., Torchia, D. F. D. O., and Castro, R. N. (2022). Humic acids affect photosynthetic quantum efficiency in rice under water deficit. Theoretical and Experimental Plant Physiology, 1-21.
45.
Wasaya, A., Shahzad Shabir, M., Hussain, M., Ansar, M., Aziz, A., Hassan, W., and Ahmad, I. (2017). Spraying of zinc and boron improved the productivity and net returns of maize grown under rainfed conditions of Pothwar plateau. Journal Of Soil Science and Plant Nutrition, 17(1), 33-45.
46.
Yang, F., Tang, C., and Antonietti, M. (2021). Natural and artificial humic substances to manage minerals, ions, water, and soil microorganisms. Chem. Soc. Rev. 50, 6221–6239. doi: 10.1039/D0CS01363C
47.
Yavas, I., and Unay, A. (2016). Effects of zinc and salicylic acid on wheat under drought stress. JAPS J. Anim. Plant Sci. 26.
48.
Zoz, T., Steiner, F., Fey, R., Castagnara, D. D., and Seidel, E. P. (2012). Response of wheat to foliar application of zinc. Ciência Rural, 42, 784-787.
