Study of the effect of alcoholic extract of prickly pear fruit on the enzymes lipooxygenase and lipase purified from the blood serum of patients with atherosclerosis and in the blood serum of male rats with neo-atherosclerosis
DOI:
https://doi.org/10.58916/jhas.v9i5.559الكلمات المفتاحية:
Atherosclerosis، Prickly pear، Lipoxygenase، Lipase، Flavonoidsالملخص
Lipoxygenase is an enzyme that catalyzes the oxidation of polyunsaturated fatty acids, and the final product of this oxidation is hydrogen hydroperoxides, which have a major role in inflammation. Lipase is an enzyme that regulates the metabolism of triglycerides in adipose tissue and blood. In this study, lipoxygenase and lipase were purified from the blood of the atherosclerosis patients by using several techniques, such as precipitation with saturated ammonium sulfate salt, dialysis, and ion exchange chromatography using DEAE-cellulose and CM-cellulose, respectively, for the two enzymes. One peak was obtained for both enzymes.
The Molecular weight of two enzymes is approximately (268.5 KDa ) , (91.7 KDa) of the lipoxygenase and lipase respectively .
The specific activity of the lipoxygenase enzyme was 0.249, 0.608, 3.329, and 12.19 enzyme units/mg protein, respectively, with the number of purification times (2.44, 13.36, 48.95) respectively. The specific activity of lipase was 1.54, 1.95, 2.89, and 15.84 enzyme units/mg protein, respectively. Respectively and with several purification times (1.95, 2.89, 15.84). Flavonoids were also isolated from the prickly pear extract using the solvent ethanol, and these compounds were identified and estimated by HPLC. The total flavonoids (Ferulic acid, Gallic acid, Cinnamic acid, Quercetin, Rutin, and Kaempferol) were 45.2, 88.9, 12.6, 80.6, 74.6, and 50.6. (20.7) micrograms/gram, respectively. A study of the effect of the alcoholic extract on the effectiveness of the two enzymes in rats with induced atherosclerosis showed a decrease in the level of activity of the two enzymes in rats treated with the extract compared to the control group with atherosclerosis. When studying the type of inhibition on the purified enzymes, the extract showed non-inhibitory inhibition. Competitive to the effectiveness of lipase and lipooxygenase at concentrations of 600 and 300 micrograms/microliter, respectively
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