Biological control of Nile leech in water using some plant extracts (Nicotiana tabacum, Cyclamen persicum) as an alternative to copper sulphate.
الملخص
The Nicotiana tabacum, and Cyclamen persicum share a group of substances that are repulsive and fatal to Nile leeches Limnatis nilotica , which are alkaloids. This experiment was designed to evaluate the possibility of using the roots of the cyclimn persicium plant, in which alkaloids are concentrated, and the leaves of the Nicotiana tabacum plant, and to compare them to the possibility of using them as an alternative to carcinogenic copper sulphate. Nile leeches collected from the field were exposed to cold water extract from both plants, with the exposure time set at 30 min. The effective dose of the Cyclamen persicum cold water extract began at 90g/1000ml, and the symptoms were paralysis and the relaxation of the leech suckers. The LD50 was 250g/1000ml, with an average death time of 23.18±18.41 min, while the lethal dose was 400gm/1000ml, with an average death time of 19.2±6.53min. As for the tobacco plant The effective dose was 50g/1000ml and the lethal dose was 80g/1000ml with a mean time to death of 8.4±3.13 min, while the effective dose for copper sulphate was 100g/1000ml LD50. 372 g/1000ml with an average mortality time of 4.67±2.93 and the (LD) of 600gm/1000ml with an average mortality time of 27.63±5.78.
Thus, the effectiveness of using the aqueous cold extracts of Nicotianac tabacum and Cyclamen persicumas an alternative to copper sulphate has been shown, as they can be used as a powerful and effective medicinal product to control the Nile leeches.at the water surfaces
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