Extraction of Polyhydroxybutyrates from Bacillius cereus isolated from Engine Oil-polluted Soils

Laila M Zargoun; Salih Muftah Bashir

doi:10.58916/jhas.v8i2.481

Authors

Laila M Zargoun 1Department of Medical Labs, College of Science and Medical Technology- Tripoli Author
Salih Muftah Bashir Department of Medical Labs, College of Science and Medical Technology- Tripoli Author

DOI:

https://doi.org/10.58916/jhas.v8i2.481

Keywords:

PHB, FITR, date molasses, Bacillus cerues

Abstract

In the present study a trial was carried out to isolate bacteria species from engine oil-polluted soils, the soil samples were diluted and spread on solid media at 37 ^oC incubation. The most species were then picked up from plates for identification, characterization, and storage for further use. According to the physical morphology and the shapes using gram stain the bacteria were belonged to Bacillus cerues, molecular technique using 16S rRNA gene were also carried out to confirm the types of species. The Sudan Black B was used to optimize the PHA granules in bacteria. The bacterium isolate were then grown in appropriate media, and date molasses, which was as carbon source was added to media to promote the growing cells and to accumulate PHB granules as intercellular product at different, range of temperature, pH, agitation speed, and carbon source concentration. The high level of PHB production was accumulated at 35 ^owith natural pH, and 150 rpm when date molasses was added at 15% (v/v). The PHB productions were characterized using biological techniques as FITR to confirm the functional group of PHA at range 4000-400 cm^-1.

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